Part:BBa_J147002
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter
Device composed of two modules, separated by a double terminator (BBa_B0014): First, a positively regulated Lux Promoter (BBa_R0062) drives the expression of a red fluorescent protein (BBa_E1010) with a medium ribosome binding site (BBa_B0032); second, a constitutive expression of Lux Receptor (BBa_C0062) and a green fluorescent protein (BBa_E0040) in tandem under a tet promoter (BBa_R0040) and both proteins with a weak RBS (BBa_B0033). This inducible device produces the expression of a red fluorescent protein upon the addition of lactone (C6-HSL) and the presence of the Lux Receptor in the cell.
This composite part has been used and extensively described in a paper published in Nucleic Acid Research in 2014 (open access). For further details and part experience, please freely download the paper or contact the authors. The reference and link are:
Carbonell-Ballestero, M. et al. A bottom-up characterization of transfer functions for synthetic biology designs: lessons from enzymology. Nucleic Acids Res (2014) doi: 10.1093/nar/gku964.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 740
Illegal AgeI site found at 852 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal BsaI.rc site found at 2531
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